B-hAPOE4 mice
| Strain Name | C57BL/6JNifdc-Apoetm1(APOE*4)Bcgen/Bcgen | Common Name | B-hAPOE4 mice |
| Background | C57BL/6JNifdc | Catalog number | 112564 |
|
Aliases |
APOE: AD2, LPG, APO-E, LDLCQ5 | ||
模型验证
Protein expression analysis in serum
Protein expression analysis in brain
mRNA expression analysis
Strain specific APOE expression analysis in homozygous B-hAPOE4 mice by ELISA. Serum collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H) were analyzed by ELISA with species-specific APOE ELISA kit. Mouse APOE was detectable in wild-type mice (A). Human APOE was exclusively detectable in homozygous B-hAPOE4 mice but not in wild-type mice (B).
Strain specific APOE expression analysis in homozygous B-hAPOE4 mice by ELISA. Brain collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H) were extracted and APOE was measured in the brain homogenates using species-specific APOE ELISA kit and then normalized to total protein levels. Mouse APOE was detectable in wild-type mice (A). Human APOE was exclusively detectable in homozygous B-hAPOE4 mice but not in wild-type mice (B).
Strain specific analysis of APOE4 mRNA expression in wild-type C57BL/6J mice and B-hAPOE4 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOE primers. Mouse Apoe mRNA was detectable only in wild-type mice. Human APOE4 mRNA was detectable only in homozygous B-hAPOE4 mice but not in wild-type mice. And the results were confirmed via Sanger sequencing.
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